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KMID : 1377020220190050987
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Tissue Engineering and Regenerative Medicine
2022 Volume.19 No. 5 p.987 ~ p.1000
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Lytic Bacteriophage as a Biomaterial to Prevent Biofilm Formation and Promote Neural Growth
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Liu Zi-Hao
Chiang Ming-Tse
Lin Hsin-Yi
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Abstract
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Background: Although non-lytic filamentous bacteriophages have been made into biomaterial to guide tissue growth, they had limited ability to prevent bacterial infection. In this work a lytic bacteriophage was used to make an antibacterial biomaterial for neural tissue repair.
Methods: Lytic phages were chemically bound to the surface of a chitosan film through glutaraldehyde crosslinking. After the chemical reaction, the contact angle of the sample surface and the remaining lytic potential of the phages were measured. The numbers of bacteria on the samples were measured and examined under scanning electron microscopy. Transmission electron microscopy (TEM) was used to observe the phages and phage-infected bacteria. A neuroblast cell line was cultured on the samples to evaluate the sample’s biocompatibility.
Results: The phages conjugated to the chitosan film preserved their lytic potential and reduced 68% of bacterial growth on the sample surface at 120 min (p?
Conclusion: The purified lytic phages were proven to be a highly bioactive nanomaterial. The phage-chitosan composite material not only promoted neural cell proliferation but also effectively prevent bacterial growth, a major cause of implant failure and removal.
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KEYWORD
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Lytic bacteriophage, Antibacterial biomaterial, Chitosan biopolymer, Neural cell growth
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